Effects of TPA on colony formation in Day-2 post 5-FU marrow cells
| Agent | Number of Colonies | ||
|---|---|---|---|
| Day 8 | Day 16 | ||
| None | 0 | 0 | |
| Vehicle | 0 | 0 | |
| IL-3 | 4 ± 1 | 14 ± 2 | |
| IL-3 + vehicle | 4 ± 2 | 13 ± 2 | |
| IL-3 + TPA | 10−8 M | 5 ± 2 | 18 ± 2* |
| 10−7 M | 6 ± 2 | 24 ± 1** | |
| 10−6 M | 1 ± 1 | 8 ± 2 | |
| IL-3 + OAG | 10−8 M | 2 ± 1 | 15 ± 2 |
| 10−7M | 2 ± 1 | 17 ± 2* # | |
| 10−6 M | 2 ± 1 | 17 ± 2* # | |
| 10−5 M | 2 ± 1 | 16 ± 2 | |
Colonies were counted on day 8 and day 16.
Data represent mean ± S.D. of quadruplicate dishes.
Neither TPA or OAG alone gave rise to colony growth.
* P < .05, ** P < .01 compared with IL-3 + vehicle control. ## P < .01 compared with IL-3 + TPA (10−7 M).