Compound | Kiapp (nM) |
---|---|
Rupatadine | 550 ± 21 |
WEB-2086 | 13 ± 3 |
BN-52021 | 221 ± 45 |
SCH-37370 | 22 ± 1 |
Membranes were incubated simultaneously with [3H]WEB-2086 (20 nM) and the compounds for 90 min at 25°C. EachK i app value represents the mean ± S.E. of K i values obtained from three separate experiments, each one involving eight different concentrations of the drug assayed in duplicate. The K D value used to calculate K i was 10 nM.