Table 5

Effects of 1 μM AF-DX 116 on estimates of the EC₅₀ and Hill coefficient for oxo-M-induced contractions under standard conditions and conditions of elevated cAMP

Condition	One-Site Analysis^5-a		Two-Site Analysis^5-b
	Control		AF-DX 116		Shift	High-potency EC₅₀	Low-potency EC₅₀	High-potency component
	EC₅₀	Hill coefficient	EC₅₀	Hill coefficient	Shift	High-potency EC₅₀	Low-potency EC₅₀	High-potency component
	nM		nM			nM	nM	%	g
Standard	207	1.5	571	1.7	2.8
Histamine/forsklin	16.3	1	473	1.9	25	16.3		100	1.8
Histamine/isoproterenol	125	0.5	506	1.1	4	6.9	144	39	1.6
Histamine/dopamine	32	1.1	180	1.6	5.6	8.5	57	38	0.7
Histamine/cicaprost	54	1.0	146	2.1	2.7

↵5-a Analysis by nonlinear regression (see “Methods”).
↵5-b Nonlinear regression analysis performed with shift parameters fixed to theoretical values. EC₅₀ values are listed for control curve only. All tissue was treated with 4-DAMP mustard as described in “Methods.”