TABLE 3

Summary of effects of LY593093 and reference muscarinic agonist compounds on displacement of N-methylscopalamine binding to membranes prepared from CHO cells stably expressing human M1–M5 muscarinic receptors

Nonspecific binding was measured in the presence of 1 μM atropine. K_i values were determined from the Cheng-Prusoff relationship K_i = IC₅₀/1 + [ligand]/K_d, where IC₅₀ is determined from a four-parameter fit of displacement curves, [ligand] = 1 nM [³H]NMS, and K_d is the equilibrium dissociation constant of [³H]NMS for each receptor determined by saturation binding experiments carried out by the membrane supplier. Data are expressed as the mean ± S.E.M. from n ≥ 3 separate experiments in log₁₀ molar (M) units.