Additions to the JPET authors checklist to promote experimental rigor and reproducibility
| Experimental design |
| ▪ Include details of sample size justification (power analysis) |
| ▪ Was randomization undertaken? If not, provide a valid justification |
| ▪ Was experimental blinding performed? If not, provide a valid justification |
| ▪ Was gender representation provided? If there is gender bias, provide a justification |
| Group sizes |
| ▪ Ensure “n” refers to the number of biologic samples, not technical replicates |
| ▪ Provide an exact “n” value not a range |
| Data analysis and statistics |
| ▪ A data and statistical analysis section is provided giving details |
| ▪ If ANOVA is used, a statement should be provided indicating that post hoc tests were conducted only if the data are normally distributed |
| ▪ Any statistical package or program employed, should be described along with the details of which tests were used |
| ▪ The threshold P value deemed to constitute statistical significance (α) should be defined in the Materials and Methods |
| ▪ Report 95% confidence intervals (CI) for main outcome variables in addition to P values |
| ▪ Do not report standard error means (S.E.M.) as an index of data dispersion |
| ▪ Standard deviations (S.D.) should be used for description of normally distributed data, and interquartile range (IQR) for non-normally distributed data |
| Outliers and exclusion criteria |
| ▪ Define in methods if data were excluded and why? |