Physicochemical properties of the vesicles used in calcein release, cell interaction (in vitro), and in vivo studies
Mean diameter, polydispersity index (PDI), and ζ-potential of CVs from HEK-293 cells (CVHEK-293), B16F10 cells (CVB16F10), and hCMEC/D3 cells (CVhCMEC) as well as pegylated CVB16F10 (PEG-CVB16F10) and pegylated CVhCMEC/D3 (PEG-CVhCMEC/D3). Liposomes (LIP), pegylated liposomes (PEG-LIP) (PC/PG/Chol/PEG) and targeted liposomes (t-LIP) properties are also reported.
| Vesicle Type | Mean Diameter | PDI | ζ-Potential |
|---|---|---|---|
| nm | mV | ||
| LIP | 126.6 ± 8.1 | 0.134 ± 0.001 | −15.1 ± 1.4 |
| PEG-LIP | 141.1 ± 1.1 | 0.169 ± 0.009 | −3.40 ± 0.77 |
| CVHEK-293 | 205 ± 49 | 0.375 ± 0.018 | −12.3 ± 0.42 |
| CVB16F10 | 219 ± 1.8 | 0.322 ± 0.032 | −12.27 ± 0.42 |
| PEG-CVB16F10 | 229.4 ± 9.4 | 0.318 ± 0.083 | −5.46 ± 0.56 |
| CVhCMEC/D3 | 122.1 ± 6.7 | 0.255 ± 0.013 | −13.21 ± 0.43 |
| PEG-CVshCMEC/D3 | 156.9 ± 4.3 | 0.335 ± 0.117 | −7.47 ± 0.29 |
| t-LIP | 181 ± 17 | 0.241 ± 0.051 | −1.90 ± 0.31 |