Experimental protocol
Canine fetuses received intra-amniotic injections at the specified embryonic gestational time points at a dose of 100 mg/kg EDI200. The injections were given only during the gestational period (groups IUTxE32/45, IUTxE45, and IUTxE55) and were not repeated postnatally.
| Name of group | |||||
|---|---|---|---|---|---|
| IUTXE32/45 | IUTxE45.1 | IUTx45.2 | IUTxE55 | IUTxE32 | |
| Genotype of dam | XLHED | HET | HET | XLHED | HET |
| Time of in utero injection | 32/45 | 45 | 45 | 55 | 32 |
| Genotype of offspring | XLHED | XLHED | 2 WT | XLHED | Unknown |
| 4 XLHED | |||||
| Number of offspring born | 5 | 3 | 6 | 7 | 0 |
| Number of fetuses treated | 5 | 3 | 5 | 4 | 6 |