TABLE 4

Functional activity of 5-HOB and formoterol in cell membranes isolated from rat tissues

% cAMP responses was determined relative to the Emax of formoterol in skeletal muscle membranes. The EC50 and Emax values ± S.E.M. of experiments from three independent membrane preparations, each performed with seven concentrations of compounds (10-fold dilutions) in triplicates.

cAMP Response5-HOBFormoterol
EC50EmaxEC50Emax
nM%nM%
Skeletal muscle80 ± 1792 ± 16 ± 0.3100 ± 0
Heart361 ± 17914 ± 262 ± 853 ± 4