TABLE 2

Bidirectional transport of ABCB1 and/or ABCG2 substrates for MDCKII/WT and MDCKII/hABCG2 cells

InhibitorConditionCell TypePappERcER
Apical to BasolateralBasolateral to Apical
10−6 cm/s
DigoxinNo suppressionMDCKII/WT1.04 ± 0.03126.53 ± 0.2686.30.7
MDCKII/hABCG21.18 ± 0.1745.00 ± 0.7344.2
Verapamil treatmentMDCKII/WT1.55 ± 0.07641.65 ± 0.04651.11.0
MDCKII/hABCG21.81 ± 0.09741.99 ± 0.08931.1
shRNA transfectionMDCKII/WT1.32 ± 0.02861.72 ± 0.09321.30.8
MDCKII/hABCG21.23 ± 0.2901.26 ± 0.1671.0
OlaparibNo suppressionMDCKII/WT2.75 ± 0.41427.3 ± 2.729.90.9
MDCKII/hABCG22.73 ± 0.46425.1 ± 4.569.2
Verapamil treatmentMDCKII/WT8.16 ± 0.16413.3 ± 0.5141.63.2
MDCKII/hABCG24.40 ± 0.38622.5 ± 1.995.1
shRNA transfectionMDCKII/WT9.35 ± 0.8449.83 ± 0.4001.14.9
MDCKII/hABCG24.02 ± 0.43520.8 ± 0.8525.2
IrinotecanNo suppressionMDCKII/WT0.611 ± 0.3644.47 ± 0.5737.33.0
MDCKII/hABCG20.404 ± 0.1478.95 ± 0.28022.1
Verapamil treatmentMDCKII/WT1.60 ± 0.2572.34 ± 0.1501.514.3
MDCKII/hABCG20.411 ± 0.1508.62 ± 0.37721.0
shRNA transfectionMDCKII/WT1.65 ± 0.4551.78 ± 0.3601.113.5
MDCKII/hABCG20.423 ± 0.0456.16 ± 0.26314.6
TopotecanNo suppressionMDCKII/WT1.06 ± 0.05214.10 ± 0.3813.95.6
MDCKII/hABCG20.452 ± 0.08809.84 ± 0.57021.8
Verapamil treatmentMDCKII/WT1.67 ± 0.1151.86 ± 0.1341.113.1
MDCKII/hABCG20.711 ± 0.14010.3 ± 0.48714.5
shRNA transfectionMDCKII/WT1.59 ± 0.1551.06 ± 0.1790.713.5
MDCKII/hABCG20.618 ± 0.2215.53 ± 0.4499.0
PrazosinNo suppressionMDCKII/WT16.3 ± 0.52716.2 ± 0.6831.09.6
MDCKII/hABCG22.76 ± 0.11026.1 ± 1.199.5
Verapamil treatmentMDCKII/WT17.3 ± 1.9114.6 ± 0.8800.812.5
MDCKII/hABCG22.66 ± 0.12628.0 ± 1.4410.5
shRNA transfectionMDCKII/WT16.3 ± 0.92815.0 ± 0.1210.99.9
MDCKII/hABCG23.96 ± 0.069235.8 ± 1.869.1