TABLE 2

Kinetic parameters for luciferin-4A O-demethylation catalyzed by HLMs, HRMs, and recombinant CYP4A11

Data points were fitted to the Hill and Michaelis–Menten (MM) models. Values of V_max, S₅₀ (substrate concentration to give 50% of V_max)/K_m, and n (Hill coefficient for cooperative binding) are means ± S.E. of kinetic parameters.

Enzymes	Models	V_max	S₅₀/K_m	n	R²^{^a}
		(pmol/min per milligram protein)	(μM)
HLMs	Hill	39.7 ± 1.3	43.2 ± 3.2	1.24 ± 0.09	0.992
	MM	44.2 ± 1.2	54.9 ± 4.1	—	0.987
HRMs	Hill	39.4 ± 1.3	33.8 ± 2.7	1.34 ± 0.13	0.977
	MM	42.5 ± 1.4	39.2 ± 4.3	—	0.963
		(pmol/min per nanomoles P450)
CYP4A11	Hill	656 ± 14	29.1 ± 1.5	1.23 ± 0.08	0.993
	MM	705 ± 13	33.6 ± 2.1	—	0.988

↵^a Coefficient of determination.