TABLE 3

PK parameters for FS315-hFc variants

CD-1 female mice (n = 3 per group) were given a single dose of 1 mg/kg by intravenous administration. Serum concentrations of the proteins were determined using an electrochemiluminescent immunoassay, and noncompartmental PK analysis PK parameters were calculated. Statistical parameters S.E. and %CV as calculated by the sparse sampling method are shown for AUC.

FS315-hFc VariantT1/2AUCCL Vss
MeanS.E.%CV
hh*ug per milliliterml/h per kilogramml/kg
Wild type68.029.80.82.730.01600
ΔHBS92.1401.022.25.51.3158
K82E93.558.14.78.112.0807
K82T58.951.01.52.918.8515
K(81,82)A80.1114.03.93.48.0385
K(81,82)E73.5264.022.08.32.7183
K(76,82)E95.4217.016.17.42.7222
K(76,81,82)A60.4169.07.84.64.5205
K(76,81,82)E154.0504.014.42.81.2227
K(76,81,82)D85.4469.010.72.31.7168
K75N/C77T/K82T55.6493.029.25.90.971
C66A/K75N/C77T51.6300.020.46.83.0194
  • T1/2, terminal half-life.