TABLE 4

Characterization of endogenous and hyperglycosylated N-linked glycosylation sites

Percentage glycan occupancy and sialic acid content are shown.

FS315-hFc VariantHyperglycosylation SiteN95N112N259
LocationOccupancySialic ContentaOccupancySialic ContentaOccupancySialic ContentaOccupancySialic Contenta
Wild type14.7%2.313.6%0.996.0%1.3
ΔHBS22.7%1.820.2%1.798.8%1.7
K82TN80N.D.N.D.49.7%1.716.2%0.897.1%1.2
P85TN83N.D.N.D.9.3%2.418.6%1.197.0%1.3
R78N/N80TN78N.D.N.D.31.3%2.417.7%1.097.0%1.4
R86N/V88TN86bb21.0%b1.0b15.1%1.096.7%1.1
G74N/K76T/P85TN74N.D.N.D.15.0%2.223.1%1.395.4%1.3
N83N.D.N.D.
K75N/C77T/K82TN7569.7%2.047.9%2.316.9%1.597.0%1.4
N80N.D.N.D.
C66A/K75N/C77TN7539.6%1.932.8%2.317.8%1.494.9%1.3
C66S/K75N/C77TN7521.5%2.032.9%2.419.7%1.495.1%1.3
  • N.D., not determined.

  • a mol sialic acid per mol oligosaccharide.

  • b Results do not distinguish glycosylation at N86 or N95.