TABLE 2

Effects of glucose and galactose on mitochondrial function in HepaRG cells

HepaRG cells were cultured in a Williams’ E medium with 11.1 mM glucose or without glucose but supplemented with 11.1 mM galactose. After 14 days, mitochondrial respiration was measured for each culture condition (i.e., glucose or galactose) in the presence of l-glutamine/pyruvate/glucose or l-glutamine/pyruvate/galactose with glucose and galactose at the same concentration (10 mM). Three mitochondrial parameters (i.e., respiration rates in nanomoles per minute per 106 cells) provided by the Mito Stress Test profile are shown. Results are mean ± S.E.M. for three independent cultures.

Substrate for CultureSubstrate for RespirationMito Stress Test Profile Parameters
Basal RespirationMaximal RespirationATP Production
GlucoseGlucose3.7 ± 0.217.9 ± 1.72.8 ± 0.3
GalactoseGlucose3.7 ± 0.317.5 ± 2.02.8 ± 0.3
GlucoseGalactose3.6 ± 0.216.8 ± 2.12.8 ± 0.2
GalactoseGalactose3.8 ± 0.215.0 ± 1.72.9 ± 0.1