Conditions for binding and uptake assays
| Assays | Species | Tissue | [3H]Ligand | Nonspecific Ligand | Reaction Conditions |
|---|---|---|---|---|---|
| (nM) | (μM) | ||||
| SERT binding | Rat | Frontal cortex | Paroxetine (0.5) | Paroxetine (10) | 23°C, 60 min |
| NET binding | Rat | Frontal cortex | Nisoxetine (0.5) | Desipramine (10) | 25°C, 30 min |
| DAT binding | Rat | Striatum | WIN35428 (0.5) | Nomifensine (10) | 4°C, 120 min |
| 5-HT uptake | Rat | Frontal cortex | 5-HT (20) | Paroxetine (10) | 37°C, 10 min |
| NE uptake | Rat | Frontal cortex | NE (20) | Desipramine (10) | 37°C, 10 min |
| DA uptake | Rat | Striatum | DA (20) | Nomifensine (10) | 37°C, 10 min |