Protein binding data for experiments 1 and 2
Drug concentration in serum (oxycodone) 4 minutes after intravenous administration of a low or high opioid dose. *P < 0.05 for the difference between vaccine and control groups given the same dose. Unpaired t tests were used to compare groups. Mean ± S.D., n = 6–8/group.
| Oxycodone Dose | Drug Concentration in Serum | ||
|---|---|---|---|
| Total | Unbound | Bound | |
| μmol/kg (mg/kg) | μM | μM | % |
| KLH | |||
| 0.7 (0.22) | 0.21 ± 0.04 | 0.17 ± 0.03 | 18.8 ± 2.5 |
| 7.0 (2.2) | 2.85 ± 0.43 | 2.37 ± 0.26 | 15.1 ± 15.2 |
| OXY-KLH | |||
| 0.7 (0.22) | 5.97 ± 1.97* | 0.10 ± 0.10* | 97.9 ± 2.1* |
| 7.0 (2.2) | 9.63 ± 2.48* | 2.34 ± 0.51 | 74.1 ± 9.8* |