Enzyme | Substrate | α-KG Km (nM) | IC50 ± S.D. (nM) | Ki app (nM) | n | Assay Format |
---|---|---|---|---|---|---|
PHD1 | HIF1α CODDD | 71 ± 12a | 3.5 ± 0.6 | 1.8 ± 0.3 | 2 | FRET |
PHD2 | HIF1α CODDD | 188 ± 14a | 22.2 ± 13.4 | 7.3 ± 4.5 | 10 | FRET |
PHD3 | HIF2α CODDD | 7500 ± 2000a | 5.5 ± 5.1 | 1.8 ± 1.7 | 10 | FRET |
FIH | HIF-1α (D788-L822) | 32 ± 6 | 9800 ± 7500 | 3200 ± 2500 | 2 | 14CO2 capture |
CP4H | Procollagen peptide | 53 ± 1 | >200,000 | >63,000 | 7 | HPLC/14CO2 |
HPLC, high-performance liquid chromatography.
↵a Reported in Pappalardi et al. (2011).