Summary of epitope mapping
| Clone | Subclass | Human CLDN-5/CLDN-1 Chimera Mutant | Human/Mouse Chimera CLDN-5 Mutant | |||
|---|---|---|---|---|---|---|
| 1–5 | 5–1 | D68E | T75A | S151T | ||
| M11 | Mouse IgG2b | − | +++ | − | +++ | +++ |
| M48 | Mouse IgG3 | +++ | − | +++ | +++ | − |
| R2 | Rat IgG2a | − | +++ | − | +++ | +++ |
| R9 | Rat IgG2b | +++ | − | +++ | +++ | + |
+ ++, +, and − indicate strong, weak, and negligible binding reactivity of anti–CLDN-5 antibody against each chimera CLDN.