TABLE 2

Human APP constructs used in cell culture experiments

ConstructAPP Sequencea at BACE1 Cleavage SiteAβ Peptide Released after BACE1 CleavageUsed in Cell Culture Experiments
hAPPwt593 EVKM/DAEFRH DSGYEVHHQKL VFFAEDVGSN KGAIIGLMVG GVV… 635Aβ(1-40) DAEFRHDSGY EVHHQKLVFF AEDVGSNKGA IIGLMVGGVV
APP-NLE593 EVNL/EFRHD SGYEVHHQKLV FFAEDVGSNK GAIIGLMVGG VV… 635Aβ(3-40) EFRHDSGY EVHHQKLVFF AEDVGSNKGA IIGLMVGGVVX
APP-NLQ593 EVNL/QFRHD SGYEVHHQKLV FFAEDVGSNK GAIIGLMVGG VV… 635Aβ(Q3-40) QFRHDSGY EVHHQKLVFF AEDVGSNKGA IIGLMVGGVVX
  • a Numbering refers to human APP695 wt variant, Frame and slash: BACE1 cleavage site; bold: amino acid exchange compared with wild-type (wt) sequence.