Effects of MNG on CFTR chloride channel activity evaluated using Ussing chambers assay.
Short-circuit current was changed dose-dependently by treatment with MNG (50–400 μg/mL), and the response was blocked by CFTRinh-172 (20 μmol/L).
| Test Article (μg/mL) | Short-Circuit Current (μA/cm2) |
|---|---|
| Vehicle | 0.02 ± 0.13 |
| +CFTRinh-172 | 16.81 ± 1.31 |
| MNG 50 | −3.04 ± 0.26*** |
| MNG 100 | −5.21 ± 0.44*** |
| MNG 200 | −6.73 ± 0.32*** |
| MNG 400 | −9.85 ± 0.17*** |
| +CFTRinh-172 | 23.10 ± 0.69 |
↵*** P < 0.01 versus vehicle by Dunnett’s test. Data are mean ± S.E.M. (n = 3).