TABLE 1

EC50 and relative efficacy values for BDNF, AB2, and AB20 in the indicated TrkB signaling assays and using the indicated model cells

The EC50 values of BDNF, AB2, and AB20 are given in ng/ml and in pM. The 95% confidence intervals (95% CI) of the EC50 values are indicated. Assays in the CHO-TrkB cells and in the GRINCH neurons were carried out using n = 8 and n = 4 replicates, respectively.

CHO-TrkBGRINCH Neurons
pTrkBpTrkBpERKpAKTpCREBVGF
BDNFEC50 (ng/ml)150.670.200.330.130.41
95% CI(ng/ml)13; 160.58; 761.6; 2.60.26; 0.420.13; 0.160.31; 0.55
EC50 (pM)540257.4124.715
Efficacy (%)100*100*100*100*100*100*
S.E.M. (%)1.12.12.83.02.43.4
AB2EC50 (ng/ml)59105.14.61.12.5
95% CI (ng/ml)50; 687.0; 153.9; 6.72.8; 7.80.58; 1.92.1; 2.9
EC50 (pM)3906734317.017
Efficacy (%)285179102108101
S.E.M. (%)1;94.74.37.37.32.5
AB20EC50 (ng/ml)4048043376711
95% CI (ng/ml)340; 48057; 11032; 5730; 4649; 928.6; 15
EC50 (pM)270053029025045073
Efficacy (%)32467291182113
S.E.M. (%)2.57.03.72.63.05.1
  • Relative efficacy values and the respective S.E.M. values are given in %, normalized to BDNF as a full agonist with 100%, as indicated by the asterisk *.