TABLE 3

Specific peptide sequence for detection of surrogate proteins and MRM conditions

ProteinSpecies 
Cross-ReactivityPeptide SequenceQ1Q3Declustering Potential Collision EnergyCell Exist Potential
m/zm/zVeVV
CYP7A1/Cyp7a1Human/mouse/ratLSSASLNIR480.8760.4662332
CYP7A1HumanAHILNNLDNFK433.4523.1441520
Cyp7a1Mouse/ratLFAVQEIK474.2616.2662326
Oatp1b2Mouse/ratSVQPELK400.7614.3801726
NTCP/NtcpHuman/mouse/ratGIYDGDLK440.8710.2801726
BSEP/BsepHuman/mouse/ratSTALQLIQR515.4657.5902526
BCRP/BcrpHuman/mouse/ratSSLLDVLAAR522.9644.31002526
P-gpHuman/mouse/ratAGAVAEEVLAAIR635.3971.4953125
MRP2/Mrp2Human/ratLTIIPQDPILFSGSLR885.8665.5903725
MRP3/Mrp3HumanIDGLNVADIGLHDLR541.6697.2902018
MRP4/Mrp4Human/mouse/ratAPVLFFDR482.8697.4662821