TABLE 3

Contraction force (mN) and slope values of different protocols

Comparison of the contraction force (mN) and slope values of the contractions were obtained using four experimental protocols. Histamine (3 × 10-6 M; protocol 1) and methacholine (5 × 10-7 M; protocol 3)-induced tracheal contraction in normal Krebs-Henseleit (KH) solution. CaCl2-induced contraction of intracellular calcium predepleted tracheal preparations in histamine (protocol 2) or methacholine (protocol 4) containing Ca2+-free KH solution.a b

Protocol 1Protocol 2Protocol 3Protocol 4
Contraction force9.6 ± 2.810.2 ± 2.811.3 ± 2.510.9 ± 2.3
Slope (×10−3)19.9 ± 7.912.7 ± 6.015.8 ± 5.2914.5 ± 6.20
  • a Values represent the mean ± S.D.

  • b n = 36 (histamine), n = 24 (methacholine).