Effect of ouabain and Akt inhibitor on Ca2+ transients in adult zebrafish heart
The experiments were performed as described in the legend to Fig. 10. Average of Ca2+ transient were measured in 10 isolated zebrafish heart in each group.
| Control | Ouabain 200 μM | MK-2206 (1 nM) | Ouabain & MK-2206 | |
|---|---|---|---|---|
| Rate (wave/min) | 58 ± 7.4 | 52 ± 6.8 | 48 ± 4.8 | 48 ± 5 |
| Amplitude (Maximal deflection from baseline) | 5.28 ± 0.32 | 6.59 ± 0.71* | 4.8 ± 0.13 | 5.91 ± 0.28*# |
| Area (Area under the transient relative to baseline) | 2.52 ± 0.28 | 2.94 ± 0.44* | 2.81 ± 0.19 | 2.08 ± 0.23 |
| Rise slope (+Δ Fluorescence in the linear phase/sec) | 4.85 ± 1.29 | 7.84 ± 2.02* | 1.65 ± 0.28* | 2.68 ± 0.34*# |
| Decay slope (-Δ Fluorescence in the linear phase/sec) | 10.73 ± 7.54 | 20 ± 3.51* | 4.65 ± 0.54 | 7.87 ± 1.11 |
| Acc mutant | ||||
| Rate (wave/min) | 31 ± 5 | 35 ± 8.4 | 25 ± 3 | 31 ± 4.4 |
| Amplitude (Maximal deflection from baseline) | 1.79 ± 0.11 | 1.67 ± 0.15* | 1.85 ± 0.05 | 1.76 ± 0.05 |
| Area (Area under the transient relative to baseline) | 2.64 ± 0.65 | 2.54 ± 0.56 | 2.79 ± 0.23 | 2.92 ± 0.4 |
| Rise slope (+Δ Fluorescence in the linear phase/sec) | 0.48 ± 0.07 | 0.47 ± 0.1 | 0.51 ± 0.04 | 0.56 ± 0.05 |
| Decay slope (-Δ Fluorescence in the linear phase/sec) | 1.88 ± 0.33 | 1.92 ± 0.46 | 1.72 ± 0.22 | 1.61 ± 0.19 |
↵* Significantly higher than control P < 0.05; #significantly higher than MK-2206 P < 0.05 (post hoc Bonferroni-adjusted between subjects ANOVA).