Potency (pEC50) and maximal response (Emax) of A2BAR-mediated changes in cAMP accumulation in the presence and absence of ADA in A2BAR-FlpInCHO cells
Data are the mean ± S.E.M. from four or more independent experiments performed in triplicate.
| pEC50 | Emax | |||
|---|---|---|---|---|
| 0 U/ml ADA | 1 U/ml ADA | 0 U/ml ADA | 1 U/ml ADA | |
| NECA | 8.42 ± 0.25 | 8.49 ± 0.19 | 1.90 ± 0.13 | 2.36 ± 0.28 |
| Adenosine | 6.29 ± 0.37 | ND | 2.40 ± 0.23 | ND |
| ZM241385 | 7.80 ± 0.25 | 8.00 ± 0.26 | 0.36 ± 0.06 | 0.51 ± 0.06 |
| PSB-603 | 8.26 ± 0.22 | 8.33 ± 0.38 | 0.40 ± 0.05 | 0.48 ± 0.04 |
Emax, maximal response elicited by the ligand expressed as cAMP concentration as fold over basal; ND, value could not be determined; pEC50, negative logarithm of the agonist or inverse agonist concentration required to elicit half the maximal response.