TABLE 1

Effects of atropine (1 µM) on electrically and carbachol-evoked cholinergic mechanical responses in isolated colonic tissues

ES-evoked cholinergic responses were recorded in tissues maintained in Krebs’ solution containing L-NAME (100 μM), guanethidine (10 μM), L-732,138 (10 μM), GR159897 (1 μM), and SB218795 (1 μM). Carbachol-induced motor responses were recorded in tissues maintained in Krebs’ solution containing tetrodotoxin (1 μM). The effects of atropine were expressed as percentage of reductions versus contractions recorded in the absence of atropine. Each number represents the mean ± S.E.M. value obtained from five to six animals (statistics: one-way analysis of variance followed by post-hoc analysis with Student-Newman-Keuls test).