Summary of kinetic constants of TNIK substrate peptides
All peptides followed Michaelis-Menten kinetics in the ADP-Glo assay. Alanine substitutions are shown in bold in the left column.
| 20-mer peptide | Km | Vmax | 1000×Vmax/Km |
|---|---|---|---|
| µM | µmol/min per mg | ||
| …TIENTRR… (SMAD1) | 46.9 ± 4.6 | 0.65 ± 0.02 | 13.9 |
| …TAENTRR… | ADP level too low to calculate kinetic constant | ||
| …TIANTRR… | 27.1 ± 4.9 | 0.75 ± 0.04 | 27.7 |
| …TIEATRR… | 61.2 ± 14.5 | 0.81 ± 0.08 | 13.3 |
| …TIENTRA… | 48.6 ± 12.3 | 0.31 ± 0.03 | 6.3 |
| …TLTR… (p120-cat) | 25.5 ± 1.6 | 1.72 ± 0.03 | 67.6 |
| …TATR… | 71.8 ± 9.4 | 1.13 ± 0.07 | 15.8 |
| …TLAR… | 27.5 ± 2.0 | 2.08 ± 0.05 | 75.5 |
| …TLTA… | 54.3 ± 7.1 | 0.99 ± 0.05 | 18.2 |
| …TLPLIDR… (p120-cat) | 147.1 ± 24.7 | 1.90 ± 0.14 | 12.9 |
| …TAPLIDR… | ADP level too low to calculate kinetic constant | ||
| …TLALIDR… | 90.7 ± 4.5 | 2.04 ± 0.04 | 22.4 |
| …TLPAIDR… | 113.3 ± 6.9 | 2.39 ± 0.06 | 21.1 |
| …TLPLIDA… | 231.5 ± 36.6 | 1.10 ± 0.09 | 4.7 |
| …TIER… (δ-cat) | 20.4 ± 2.3 | 1.47 ± 0.05 | 72.1 |
| …TAER… | ADP level too low to calculate kinetic constant | ||
| …TIAR… | 29.8 ± 3.3 | 2.03 ± 0.07 | 68.0 |
| …TIEA… | ADP level too low to calculate kinetic constant | ||