TABLE 2

In vivo cystometry in awake rats: effect of intravenous DFL23448 (10 mg/kg) or vehicle on cold stress–induced bladder overactivity by skin application of menthol (50% solution)

Values are given as means ± S.E.M. (numbers are individual animals).

Treatment (Intravenous)	Intervals and Volumes				Pressures
Treatment (Intravenous)	Micturition Interval	Micturition Volume	Residual Volume	Bladder  Capacity	Basal Pressure	Threshold Pressure	Maximum Pressure
	min	ml			cmH₂O
Vehicle (n = 6)
Baseline	3.64 ± 0.75	0.59 ± 0.12	0.02 ± 0.01	0.59 ± 0.12	11.9 ± 0.9	18.0 ± 0.9	69.8 ± 2.8
Menthol	2.60 ± 0.63**^,§	0.37 ± 0.11**′^§	0.06 ± 0.01*′^§	0.43 ± 0.10**′^§	15.6 ± 1.8*′^§	24.5 ± 2.0*′^§	59.9 ± 3.7**
10 mg/kg DFL23448 (n = 8)
Baseline	4.92 ± 0.64	0.87 ± 0.14	0.02 ± 0.01	0.89 ± 0.13	8.9 ± 0.6	14.2 ± 0.9	46.2 ± 2.9
Menthol	5.47 ± 0.66*	1.01 ± 0.14*	0.01 ± 0.01	1.02 ± 0.14*	7.9 ± 0.3*	12.7 ± 0.6*	50.0 ± 2.6*

* P < 0.05 (paired t test for comparisons within groups); **P < 0.01 (paired t test for comparisons within groups); ^§P < 0.05 (Newman-Keuls analysis of variance for comparisons between groups).