Primers used in this study for mutagenesis, qPCR, and cloning (5′–3′)
| Primers | Sequence (5′–3′) |
|---|---|
| Site-directed mutagenesis | |
| 2B15_miR376c MT_F | CTTTAGCTGAATTATCGGTTGTCAATGATTTTTAAGC |
| 2B15_miR376c_MT_R | GCTTAAAAATCATTGACAACCGATAATTCAGCTAAAG |
| 2B17_miR376c_MT_F | CTTTAGTTGGAATTATCGACTGTCAATGATTTTTAAGC |
| 2B17_miR376c_MT_R | GCTTAAAAATCATTGACAGTCGATAATTCCAACTAAAG |
| miRNA-specific qPCR | |
| hsa-miR-376c_F | CGCAGAACATAGAGGAAATTCC |
| hsa-miR-376_R | GGTCCAGTTTTTTTTTTTTTTTCGT |
| RNU6-2_F | CGCAAGGATGACACGCAA |
| RNU6-2_R | CAGGTCCAGTTTTTTTTTTTTTTTAAAAATAT |
| Primary miRNA-specific qPCR | |
| pri-miR-376c_F | GGTATTTAAAAGGTGGATATTCCTTCTATG |
| pri-miR-376_R | GATACTGAAAACGTGGAATTTCCTCTATG |
| RNU6-2_F | CGCAAGGATGACACGCA |
| RNU6-2_R | AAAAATATGGAACGCTTCAC |