Effect of oral GSK2041706 and metformin alone or in combination on number of c-fos–positive nuclei in overnight fasted DIO mice
Data are mean ± S.E.M. Overnight fasted DIO mice were orally dosed at 10:30 AM, and brains were collected 2.5 hours postdosing. n = 4 mice/group.
| Treatment | AP | NTS | PVN | VMH | LH |
|---|---|---|---|---|---|
| Vehicle | 7.75 ± 2.59 | 23.75 ± 12.69 | 57.5 ± 14.82 | 69 ± 26.5 | 60.00 ± 20.45 |
| GSK2041706 (30 mg/kg) | 8.75 ± 4.13 | 5.00 ± 2.04 | 26.0 ± 3.72 | 93.5 ± 13.29 | 62.25 ± 12.66 |
| Metformin (30 mg/kg) | 61.25 ± 21.51* | 72.75 ± 41.88 | 84.25 ± 32.89 | 106.75 ± 10.45 | 77.25 ± 11.56 |
| Metformin (100 mg/kg) | 41.50 ± 6.98* | 114 ± 27.36* | 305.75 ± 42.03* | 133.50 ± 23.95 | 51.50 ± 17.00 |
| GSK2041706 + metformin (30 mg/kg) | 42.25 ± 5.34* | 35.00 ± 3.76 | 61.25 ± 15.91 | 145.25 ± 7.85* | 77.00 ± 14.59 |
| GSK2041706 + metformin (100 mg/kg) | 17.57 ± 6.25 | 107.75 ± 39.1 | 56.5 ± 16.81 | 112.00 ± 28.53 | 22.75 ± 3.35 |
↵* P < 0.05 versus vehicle.