Effects of R1 on the activity of MPO and the levels of TNF-α and IL-6 in mice with DSS- or TNBS-induced colitis
Colon segments from mice (n = 6 per group) were excised and homogenized. The supernatants were assayed for the determination of the activity of MPO and the levels of TNF-α and IL-6 as described in Materials and Methods. Values are expressed as the mean ± S.D.
| Group | DSS | TNBS | ||||
|---|---|---|---|---|---|---|
| TNF-α | IL-6 | MPO | TNF-α | IL-6 | MPO | |
| pg/mg protein | U/mg protein | pg/mg protein | U/mg protein | |||
| Vehicle | 14.6 ± 1.7 | 28.9 ± 2.3 | 3.7 ± 0.6 | 27.4 ± 1.2 | 39.7 ± 3.3 | 2.8 ± 0.2 |
| R1 | 16.96 ± 1.1 | 23.79 ± 4.1 | 3.2 ± 0.2 | 30.9 ± 3.6 | 35.8 ± 1.9 | 3.1 ± 0.3 |
| DSS/TNBS | 127.4 ± 8.5 | 214.3 ± 17.9 | 26.8 ± 2.2 | 86.6 ± 6.4 | 119.7 ± 7.9 | 15.3 ± 1.7 |
| DSS/TNBS + R1 | 61.5 ± 3.9** | 164.2 ± 16.6* | 11.4 ± 1.8** | 52.2 ± 4.1** | 86.3 ± 7.4* | 8.8 ± 0.7* |
↵* P < 0.05; **P < 0.01 versus DSS- or TNBS-treated group.