Sequences of the sense and antisense primers for rat ClC-2, EP1–EP4 receptors, iNOS, and TNFα.
| Gene | Primer Sequence 5′–3′ | PCR Product |
|---|---|---|
| bp | ||
| CIC2 | 499 | |
| Forward | CAAGTTCCTCTCCCTCTTTG | |
| Reverse | GAACTGTCCAAAGCCAGGG | |
| EP1 | 778 | |
| Forward | CCCAGGGTCCCCAATACATCT | |
| Reverse | GGGCAGCTGTGGTTGAAG | |
| EP2 | 1178 | |
| Forward | CGCCCTCCACCATGGACAAT | |
| Reverse | AAGCAGCGCATGCTCACAAC | |
| EP3 | 666 | |
| Forward | TGGCTGGCGCTCACCGACTTG | |
| Reverse | GCATTGCTCTACTGACATCTG | |
| EP4 | 488 | |
| Forward | CCCTGCAGCGCCTCAGTGACTTT | |
| Reverse | CTTGCCTCCGAGGCTGCTTTCAGT | |
| TNF-α | 331 | |
| Forward | GTCCCAACAAGGAGGAGAAGTT | |
| Reverse | TCCTGGTATGAAGTGGCAAATC | |
| iNOS | 780 | |
| Forward | CGGTTCACAGTCTTGGTGAAAG | |
| Reverse | CAGGTGTTCCCCAGGTAGGTAG | |
| GAPDH | 331 | |
| Forward | GAACGGGAAGCTCACTGGCATGGC | |
| Reverse | TGAGGTCCACCACCCTGTTGCTG |