Analysis of expression of TRAIL receptors in A549 and A549/Pt cells after exposure to ATO and sanguinarine
Drug-induced expression levels of TRAIL-R1 (DR4) and TRAIL-R2 (DR5) were determined by cytofluorometric analysis after 24-hour exposure to arsenic trioxide (10 and 30 μM in A549 and A549/Pt cells, respectively), sanguinarine (1 and 2 μM in A549 and A549/Pt cells, respectively), or to the combined treatment. Results on modulation of TRAIL receptor expression are expressed as the mean fluorescence intensity (MFI). MFI values (± S.D.) from three experiments are reported. Control refers to cells incubated with isotypic control.
| MFI | |||
|---|---|---|---|
| Control | TRAIL-R1 | TRAIL-R2 | |
| A549 | |||
| Untreated | 4.15 ± 0.9 | 155.8 ± 5.5 | 168.60 ± 8.2 |
| ATO | 5.14 ± 0.9 | 146.9 ± 15.6 | 156.70 ± 19.8 |
| S | 8.18 ± 0.3 | 168.40 ± 22.9 | 150.20 ± 5.2 |
| S+ATO | 7.04 ± 1.0 | 143.80 ± 16.05 | 645.50 ± 76.9* |
| A549/Pt | |||
| Untreated | 2.64 ± 0.3 | 34.44 ± 2.8 | 61.74 ± 5.3 |
| ATO | 2.87 ± 0.4 | 34.77 ± 4.1 | 206.20 ± 15.6* |
| S | 5.3 ± 0.8 | 48.80 ± 5.1 | 62.11 ± 3.5 |
| S+ATO | 4.57 ± 0.4 | 19.14 ± 6.7 | 174.90 ± 5.08* |
↵* P < 0.05, unpaired t test of control versus treated A549 cells.