NHE1inhibition by LTA (10 µg/ml) for 0.5 or 18 hours in the presence of the corresponding inhibitors of SRC (PP1 and PP2, 10 µM), PI3K (LY294002, 20 µM), AC3 (KH7, 300 µM), PKA (H89, 10 µM), PLC (U73,122, 3 µM), and PKC (chelerythrine, 1 µM)
All experiments were performed in the presence of S3226 (100 nM), an NHE3-selective inhibitor. Values represent means and S.E.M.
| Target | Inhibitor | Vehicle | LTA (10 µg/ml) 0.5 h | LTA (10 µg/ml) 18 h |
|---|---|---|---|---|
| Control | — | 100.00 ± 3.24 | 75.57 ± 2.86* | 83.83 ± 2.54* |
| NF-κB | PDTC (10 µM) | 95.00 ± 4.57 | 75.5 ± 4.75* | 78.8 ± 3.57* |
| NF-κB | PDTC (100 µM) | 103.90 ± 5.01 | 81.8 ± 3.14* | 82.9 ± 4.57* |
| SRC | PP1 (10 µM) | 101.40 ± 2.99 | 98.83 ± 3.88 | 100.09 ± 4.18 |
| SRC | PP2 (10 µM) | 96.79 ± 3.96 | 96.98 ± 5.20 | 103.02 ± 3.08 |
| PI3K | LY294002 (20 µM) | 98.91 ± 5.66 | 101.67 ± 2.94 | 101.69 ± 4.23 |
| AC3 | KH7 (300 µM) | 99.85 ± 3.45 | 97.65 ± 2.02 | 100.12 ± 3.57 |
| PKA | H89 (10 µM) | 100.07 ± 2.73 | 97.07 ± 3.75 | 95.65 ± 5.04 |
| PLC | U73,122 (3 µM) | 101.86 ± 2.07 | 96.26 ± 2.17 | 98.51 ± 2.26 |
| PKC | Chelerythrine (100 nM) | 98.84 ± 3.01 | 99.96 ± 3.64 | 94.19 ± 3.98 |
↵* Significantly different from corresponding vehicle values using the Newman-Keuls test.