CI | CIE | HI | HIE | |
---|---|---|---|---|
Starting body weight (g) | 22.6 ± 0.3 | 22.2 ± 0.2 | 21.7 ± 0.2 | 22.4 ± 0.1 |
Body weight increase (g) | 12.4 ± 0.8 | 10.7 ± 1.1 | 10.7 ± 0.9 | 6.8 ± 1.1a |
Liver weight (g) | 1.70 ± 0.07 | 1.53 ± 0.05 | 1.63 ± 0.04 | 1.29 ± 0.06a,b |
Liver/body weight (%) | 4.85 ± 0.10 | 4.67 ± 0.12 | 5.03 ± 0.14 | 4.43 ± 0.09b |
Serum total cholesterol (mg/dl) | 111 ± 6 | 108 ± 9 | 125 ± 4 | 98 ± 6 |
Serum triglyceride (mg/dl) | 55 ± 5 | 35 ± 3c | 29 ± 1c | 28 ± 1 |
Hepatic total cholesterol (mg/g tissue) | 3.33 ± 0.12 | 2.51 ± 0.11c | 3.52 ± 0.19 | 2.95 ± 0.15 |
Hepatic triglyceride (mg/g tissue) | 45.1 ± 4.5 | 42.3 ± 8.7 | 49.5 ± 5.8 | 20.0 ± 4.9d |
Hepatic mRNA expression of lipid metabolism-related genes (fold change of CI) | ||||
Srebp2 | 1.00 ± 0.10 | 1.27 ± 0.08 | 1.63 ± 0.20e | 2.57 ± 0.13b,f |
HMG-CoA reductase | 1.00 ± 0.13 | 1.39 ± 0.14 | 2.51 ± 0.72 | 3.71 ± 0.35f |
LDL receptor | 1.00 ± 0.12 | 1.15 ± 0.09 | 1.18 ± 0.09 | 1.55 ± 0.14 |
Srebp-1c | 1.00 ± 0.11 | 0.68 ± 0.03 | 1.36 ± 0.19 | 1.26 ± 0.22 |
Fas | 1.00 ± 0.15 | 0.98 ± 0.10 | 1.08 ± 0.26 | 0.90 ± 0.05 |
Acox1 | 1.00 ± 0.14 | 1.14 ± 0.10 | 1.83 ± 0.26c | 1.70 ± 0.08 |
CI, control + iron group; CIE, control + iron + ezetimibe group; HI, high-fat diet + iron group; HIE, high-fat diet + iron + ezetimibe group.
↵a P < 0.05, significant difference from CIE.
↵b P < 0.01, significant difference from HI.
↵c P < 0.01, significant difference from CI.
↵d P < 0.05, significant difference from HI.
↵e P < 0.05, significant difference from CI.
↵f P < 0.01, significant difference from CIE.