TABLE 3

Body weight, liver weight, liver weight to body weight, serum and hepatic lipid profiles, and mRNA expression of lipid metabolism related genes in liver in experiment 2

Data are presented as mean ± S.E.M. (each group, n = 5 or 6 animals).

CICIEHIHIE
Starting body weight (g)22.6 ± 0.322.2 ± 0.221.7 ± 0.222.4 ± 0.1
Body weight increase (g)12.4 ± 0.810.7 ± 1.110.7 ± 0.96.8 ± 1.1a
Liver weight (g)1.70 ± 0.071.53 ± 0.051.63 ± 0.041.29 ± 0.06a,b
Liver/body weight (%)4.85 ± 0.104.67 ± 0.125.03 ± 0.144.43 ± 0.09b
Serum total cholesterol (mg/dl)111 ± 6108 ± 9125 ± 498 ± 6
Serum triglyceride (mg/dl)55 ± 535 ± 3c29 ± 1c28 ± 1
Hepatic total cholesterol (mg/g tissue)3.33 ± 0.122.51 ± 0.11c3.52 ± 0.192.95 ± 0.15
Hepatic triglyceride (mg/g tissue)45.1 ± 4.542.3 ± 8.749.5 ± 5.820.0 ± 4.9d
Hepatic mRNA expression of lipid metabolism-related genes (fold change of CI)
 Srebp21.00 ± 0.101.27 ± 0.081.63 ± 0.20e2.57 ± 0.13b,f
 HMG-CoA reductase1.00 ± 0.131.39 ± 0.142.51 ± 0.723.71 ± 0.35f
 LDL receptor1.00 ± 0.121.15 ± 0.091.18 ± 0.091.55 ± 0.14
 Srebp-1c1.00 ± 0.110.68 ± 0.031.36 ± 0.191.26 ± 0.22
 Fas1.00 ± 0.150.98 ± 0.101.08 ± 0.260.90 ± 0.05
 Acox11.00 ± 0.141.14 ± 0.101.83 ± 0.26c1.70 ± 0.08

  • CI, control + iron group; CIE, control + iron + ezetimibe group; HI, high-fat diet + iron group; HIE, high-fat diet + iron + ezetimibe group.

  • ↵a P < 0.05, significant difference from CIE.

  • ↵b P < 0.01, significant difference from HI.

  • ↵c P < 0.01, significant difference from CI.

  • ↵d P < 0.05, significant difference from HI.

  • ↵e P < 0.05, significant difference from CI.

  • ↵f P < 0.01, significant difference from CIE.