pBFs comparing potency for calcitonin secretion, Gq/11 signaling, and [Ca2+]i mobilization
The pBF of an agonist is calculated as the ratio between EC50 between two different signaling pathways (pathway1:pathway2). However, for analysis of statistic difference between pBF of calcium and pBFs for all other agonists the negative logarithm of pBF or ppBF(pathway1:pathway2) = pEC50(pathway1) − pEC50(pathway2) was used. Statistical comparison of ppBFs for [Sr2+]e to pBFs of [Ca2+]e was performed by using Student's t tests.
| Agonist | pBF(IP1:Calcitonin) (ppBF(IP1:Calcitonin) ± S.E.M.) | pBF([Ca2+]i:Calcitonin) (ppBF([Ca2+]i:Calcitonin) ± S.E.M.) | pBF([Ca2+]i:IP1) (ppBF([Ca2+]i:IP1)± S.E.M.) |
|---|---|---|---|
| [Ca2+]e | 2.0 (−0.295 ± 0.125) | 0.60 (0.22 ± 0.153) | 0.31 (−0.515 ± 0.089) |
| [Sr2+]e | 10 (−1.02 ± 0.128)* | 3.8 (−0.585 ± 0.138)* | 0.37 (−0.435 ± 0.053) |
↵* , p < 0.05.