pBFs comparing potency for ERK1/2 phosphorylation with calcitonin secretion, Gq/11 signaling, and [Ca2+]i mobilization
The pBF of an agonist is calculated as the ratio between EC50 between two different signaling pathways (pathway1:pathway2). However, for analysis of statistic difference between pBF of calcium and pBFs for all other agonists the negative logarithms of pBF or ppBF(pathway1:pathway2) = pEC50(pathway1) − pEC50(pathway2) were used. Statistical comparison of ppBFs for [Sr2+]e to ppBFs of [Ca2+]e was performed by using Student's t tests.
| Agonist | pBF(ERK1/2:Calcitonin) (ppBF(ERK1/2:calcitonin) ± S.E.M.) | pBF(ERK1/2:[Ca2+]i) (ppBF(ERK1/2:[Ca2+]i) ± S.E.M.) | pBF(ERK1/2:IP1) (ppBF(ERK1/2:IP1) ± S.E.M.) |
|---|---|---|---|
| [Ca2+]e | 1.2 (−0.095 ± 0.137) | 2.1 (−0.315 ± 0.105) | 0.63 (0.200 ± 0.056) |
| [Sr2+]e | 0.51 (−0.292 ± 0.190) | 0.13 (0.877 ± 0.150)** | 0.049 (1.31 ± 0.14)** |
↵** , p < 0.01.