Affinities of [3H]N-methylspiperone for wild-type and mutant D2 and D4 receptors
Binding affinities (KD) and receptor densities (Bmax) are expressed as the mean ± S.D. of three separate experiments. Fold change relative to the appropriate wild-type receptor is indicated in parentheses with down arrows (↓) indicating a decrease in KD value (higher relative affinity) or decrease in Bmax value (decreased relative receptor density) and up arrows (↑) indicating a increase in KD value (lower relative affinity) or increase in Bmax value (increased relative receptor density).
| Receptor | [3H]N-Methylspiperone | |
|---|---|---|
| Bmax ± S.D. | KD ± S.D. | |
| fmol/mg protein | pM | |
| Wild-type D2 | 8647 ± 2430 (1) | 86 ± 24 (1) |
| D2-T7.39A | 4211 ± 688 (2.1↓) | 46 ± 6 (1.9↓) |
| Wild-type D4 | 1900 ± 381 (1) | 206 ± 44 (1) |
| D4-T7.39A | 6913 ± 2854 (3.6↑) | 84 ± 12 (2.5↓) |