Sequences for the primers of quantitative real-time RT-PCR
| Gene (Accession No.) | Primers | Product Size | Tm | |
|---|---|---|---|---|
| Direction | Sequences (5′ to 3′) | |||
| bp | °C | |||
| Oct4A (NM_002701) | Forward | GTGGAGAGCAACTCCGATG | 86 | 60 |
| Reverse | TGCTCCAGCTTCTCCTTCTC | |||
| SOX2 (NM_003106) | Forward | CGAGTGGAAACTTTTGTCGGA | 74 | 60 |
| Reverse | TGTGCAGCGCTCGCAG | |||
| Nanog (NM_024865) | Forward | ATTCAGGACAGCCCTGATTCTTC | 76 | 60 |
| Reverse | TTTTTGCGACACTCTTCTCTGC | |||
| MDR1 (NM_000927) | Forward | TGGCAAAGAAATAAAGCGACTGA | 76 | 60 |
| Reverse | CAGGATGGGCTCCTGGG | |||
| ABCG2 (NM_004827) | Forward | CCCCAGGCCTCTATAGCTCAGATCA | 164 | 60 |
| Reverse | TCCACGGCTGAAACACTGCTGA | |||
| MRP (NM_004996) | Forward | CCTGGAGCTGGCCCACCTGA | 122 | 60 |
| Reverse | CCCGGGCTAGGCACACAAGC | |||
| NIS (NM_000453) | Forward | CACGCTGTACCCACCCAGCG | 174 | 60 |
| Reverse | TCAGCTAAGGCGGGTCGGCT | |||
| Thyroglobulin (NM_003235) | Forward | GGACTCAGATGCCCGCGTCG | 152 | 60 |
| Reverse | CCCAGGCACTGGCCATGTCG | |||
| Thyroperoxidase (NM_000547) | Forward | GGTCAGAATGAGAGCGCTCGCT | 178 | 60 |
| Reverse | CGTGGCGTACATGGCGGTGT | |||
| GAPDH (NM_002046) | Forward | CATCATCCCTGCCTCTACTG | 180 | 60 |
| Reverse | GCCTGCTTCACCACCTTC | |||
F, forward; R, reverse; bp, base pairs.