TABLE 3

Effects of 8-MOP on NNK-induced O6-mG adduct formation in the liver and lung of Cyp2a5-null mice

Two-month-old female Cyp2a5-null mice were pretreated with 8-MOP (at 0, 12.5, or 50 mg/kg, p.o.) in 0.2 ml of corn oil once daily for 3 consecutive days. One hour after the final injection of 8-MOP, NNK was given at a dose of 100 mg/kg i.p., and tissue levels of O6-mG and total guanine were determined at 4 h after the NNK injection. Data represent means ± S.D. (n = 4).

8-MOP DoseO6-mG Level
LungLiver
pmol/μmol guanine
0 mg/kg19.0 ± 1.8590 ± 21
12.5 mg/kg13.4 ± 1.5a337 ± 70b
50 mg/kg6.2 ± 3.0b170 ± 99b

  • ↵a p < 0.05, compared with the 0 mg/kg 8-MOP (vehicle control) group (one-way ANOVA followed by Tukey's post hoc test).

  • ↵b p < 0.01, compared with the 0 mg/kg 8-MOP (vehicle control) group (one-way ANOVA followed by Tukey's post hoc test).