Effect of BNPP (100 μM) on IC50 values of phospho-NSAIDs
The cells were preincubated with BNPP for 30 min, followed by incubation with phospho-NSAIDs for 24 h. The IC50 values were determined by the MTT assay.
| Phospho-NSAID | IC50 | CES1/Control | CES2/Control | ||
|---|---|---|---|---|---|
| HEK293-Control + BNPP | HEK293-CES1 + BNPP | HEK293-CES2 + BNPP | |||
| μM | |||||
| Phospho-sulindac | 27 ± 1 | 25 ± 3 | 0.9 | ||
| Phospho-ibuprofen | 55 ± 4 | 52 ± 3 | 0.9 | ||
| Phospho-naproxen | 56 ± 7 | 43 ± 4 | 54 ± 5 | 0.8 | 1.0 |
| Phospho-indomethacin | 41 ± 2 | 29 ± 6 | 0.7 | ||
| Phospho-tyrosol-indomethacin | 38 ± 3 | 49 ± 4 | 40 ± 2 | 1.3 | 1.0 |
| Phospho-valproic acid | 21 ± 1 | 18 ± 1 | 0.9 | ||