Comparison of results from different tests for α1 GlyR mutations M287L and Q266I
| Electrophysiology in Oocytes, Homomeric α1 GlyRs | Strychnine Binding, Spinal Cord + Brain Stem from Heterozygous Mice | Electrophysiology in Isolated Neurons, Brain Stem |
|---|---|---|
| M287L | ||
| Reduced glycine potency, increased EC50 | Reduced displacement by glycine, increased IC50 | Heterozygous: similar glycine potency, same EC50 |
| Reduced maximal glycine current amplitude | No changes in strychnine BMAX | Heterozygous: no change in maximal glycine current amplitude; homozygous: reduced maximal glycine current amplitude |
| Reduced taurine potency, increased EC50 | Reduced displacement by taurine, increased IC50 | Not tested |
| Reduced taurine efficiency, decreased IMAX | No equivalent | Not tested |
| Reduced zinc-enhacement of ethanol potentiation | Not tested | Reduced ethanol potentiation, existing zinc in the buffer |
| Q266I | ||
| Reduced glycine potency, increased EC50 | Reduced displacement by glycine, increased IC50 | Heterozygous: similar glycine potency, same EC50 |
| Reduced maximal glycine current amplitude | No changes in strychnine BMAX | Heterozygous and homozygous: reduced maximal glycine current amplitude |
| Abolished ethanol potentiation | Not tested | Heterozygous: reduced ethanol potentiation; homozygous: abolished ethanol potentiation |