Comparison of ETAR-operated Ca2+ entry mediated through wild-type and mutant TRPC6 in HEK293 cells stably coexpressing human ETAR and TRPC6 proteins
Results are presented as means ± S.E.M. of n number of experiments. Sequences represent amino acids at amino-terminal to phosphorylation sites for PKA. [Ca2+]iMAX (nM) is the peak of ROCE induced by 10 nM ET-1.
| Mutation | Sequences | n | [Ca2+]iMAX |
|---|---|---|---|
| nM | |||
| Wild type | 8 | 266.4 ± 40.1 | |
| S14A | R-R-G-G-S14 | 6 | 243.8 ± 32.6 |
| S28A | R-R-N-E-S28 | 7 | 247.4 ± 26.6 |
| T69A | R-R-Q-T69 | 7 | 282.8 ± 27.6 |
| S321A | R-K-L-S321 | 6 | 221.0 ± 38.8 |
S, serine; A, alanine; T, threonine; G, glycine; N, asparagine; E, Glutamic acid; Q, glutamine; K, lysine; L, leucine.