Effects of liver donor demographics and UGT1A1 polymorphisms on Rac-7-, R-7-, and S-7-hydroxywarfarin glucuronidation activities measured in a bank of 50 human liver microsomes
| n | Rac-7-Hydroxywarfarin Glucuronidation | R-7-Hydroxywarfarin Glucuronidation | S-7-Hydroxywarfarin Glucuronidation | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean | S.D. | p Value | Mean | S.D. | p Value | Mean | S.D. | p Value | |||
| pmol equivalents* · min−1 · mg protein−1 | |||||||||||
| Race/ethnicity | White | 44 | 78.6 | 67.9 | 0.205 | 12.0 | 10.8 | 0.653 | 8.8 | 7.4 | 0.16 |
| Black | 4 | 49.4 | 37.6 | 6.0 | 2.625 | 5.7 | 2.7 | ||||
| Hispanic | 2 | 22.4 | 21.8 | 15.1 | 13.9 | 2.0 | 1.2 | ||||
| Sex | Male | 35 | 75.6 | 69.5 | 0.738 | 11.4 | 10.6 | 0.708 | 8.6 | 7.5 | 0.569 |
| Female | 15 | 70.3 | 57.9 | 12.0 | 10.5 | 7.5 | 6.6 | ||||
| Smoking | Yes | 18 | 80.2 | 60.5 | 0.453 | 11.3 | 10.7 | 0.719 | 8.6 | 6.2 | 0.605 |
| No | 27 | 78.0 | 72.8 | 12.3 | 11.3 | 9.1 | 8.1 | ||||
| Alcohol (>14 per week) | Yes | 13 | 126.9 | 73.7 | 0.002 | 18.5 | 11.1 | 0.003 | 13.4 | 7.5 | 0.009 |
| No | 31 | 59.0 | 55.5 | 9.5 | 9.9 | 7.2 | 6.6 | ||||
| UGT1A1*28 | 6/6 | 23 | 79.4 | 63.0 | 0.165 | 11.4 | 10.4 | 0.91 | 7.1 | 6.3 | 0.236 |
| 6/7 | 16 | 81.8 | 80.1 | 12.6 | 11.8 | 10.9 | 9.1 | ||||
| 7/7 | 6 | 44.9 | 59.2 | 12.2 | 12.5 | 5.6 | 5.2 | ||||
| UGT1A1*28 (no alcohol) | 6/6 or 6/7 | 28 | 62.4 | 57.1 | 0.038 | 11.687 | 9.6 | 0.028 | 6.101 | 5.1 | 0.852 |
| 7/7 | 5 | 21.6 | 17.9 | 6.054 | 8.6 | 7.318 | 9.4 | ||||
↵* Using substrate standard curve.