TABLE 1

TAK-438 dissociation of the mutants expressed in HEK293 cells

Wild type and mutants of the rabbit gastric H,K-ATPase expressed in HEK293 cells were prepared at a concentration of 2.5 μg/ml the H,K-ATPase. Each mutant was incubated with 50 nM [14C]TAK-438, and the dissociation of TAK-438 was measured as described under Materials and Methods.

MutantHalf-Time of TAK-438 Dissociation
h
Wild type4.7
M334A4.1
M334I4.9
Y799F1.5