Sequences of primers used for the construction of plasmids
| Primer Sequence (5′ to 3′) | |
|---|---|
| hCAR-WT-LBD (Gln-105 to Ser-348) | 5′-GGA-GGA-ATT-CCA-ACT-GAG-TAA-GGA-GCA-AGA-A-3′ (forward) |
| 5′-GGG-AGG-ATC-CTC-AGC-TGC-AGA-TCT-CCT-GG-3′ (reverse) | |
| hCAR-SV23-LBD (Gln-105 to Ser-352) | 5′-GGA-GGA-ATT-CCA-ACT-GAG-TAA-GGA-GCA-AGA-A-3′ (forward) |
| 5′-GGG-AGG-ATC-CTC-AGC-TGC-AGA-TCT-CCT-GG-3′ (reverse) | |
| hSRC1-RID (Asp-621 to Asn-765) | 5′-GGA-GGA-ATT-CGA-TGG-AGA-CAG-TAA-ATA-CTC-TC-3′ (forward) |
| 5′-GGG-AGG-ATC-CTC-AGT-TTG-GAG-TTG-ATC-TTA-AAT-3′ (reverse) | |
| hSRC2-RID (Lys-583 to Thr-779) | 5′-GGA-GGA-ATT-CAA-AGA-CTG-TTT-TGG-ACT-ATA-TG-3′ (forward) |
| 5′-GGG-AGG-ATC-CTC-ATG-TGT-TAC-TGG-CAG-GAT-CTG-3′ (reverse) | |
| hSRC3-RID (Ser-582 to Asp-782) | 5′-GGA-GGG-ATC-CGT-AGT-TCA-ATG-TGT-CAG-TCA-AAT-AG-3′ (forward) |
| 5′-GGG-AAA-GCT-TTC-AGT-CTT-TCT-CTT-GAC-TTG-AGC-3′ (reverse) |