Primers for quantitative reverse transcriptase-PCR of pde4 genes and pde4b isoforms
| Primer | |
|---|---|
| Mouse Pde4a pan-specific | |
| Pde4a F | 5′-CACAGCCTCTGTGGAGAAGTC-3′ |
| Pde4a R | 5′-GTGATACCAATCCCGGTTGTC-3′ |
| Mouse Pde4b pan-specific | |
| Pde4b F | 5′-GACCGGATACAGGTTCTTCG-3′ |
| Pde4b R | 5′-CAGTGGATGGACAATGTAGTCA-3′ |
| Mouse Pde4d pan-specific | |
| Pde4d F | 5′-TGTCCACAGTCAACGCCGGGAG-3′ |
| Pde4d R | 5′-CCAAGACCTGAGCAAACGGGGTCA-3′ |
| Mouse Pde4b1, 3, 4 limited isoform-specific | |
| Pde4b1, 3, 4 F | 5′-GCCAGCTTGCGAAGTGTAAG-3′ |
| Pde4b1, 3, 4 R | 5′-TAGTTCCTCCAGCGTCTCCA-3′ |
| Mouse Pde4b2 isoform-specific | |
| Pde4b2 F | 5′-AGGTGGAGGAGACTCGGCTA-3′ |
| Pde4b2 R | 5′-CGGTTCAGCATCCTTTTGA-3′ |
| Mouse Pde4b5 isoform-specific | |
| Pde4b5 F | 5′-AGAGCTTCCAAAATGCCTGA-3′ |
| Pde4b5 R | 5′-GGATTTCCACATCGTTCTGC-3′ |
| Human PDE4B2 isoform-specific | |
| PDE4B2 F | 5′-GCGGTGACTCTGCTATGGAC-3′ |
| PDE4B2 R | 5′-CCTCCAGCGTTTCCATTGCTAA-3′ |
F, forward; R, reverse.