TABLE 2

Echothiophate challenge of wild-type mice

The 1× LD₅₀ for echothiophate was 100 μg/kg s.c. The time interval between the first and second dose of echothiophate was 3 h, and between the second and third dose was 4 h. Animals that survived three doses were treated with a fourth dose the next day, approximately 18 h after the third dose. BChE activity is the activity in plasma before treatment with the first dose of echothiophate.

Mouse	Type of Virus	BChE Activity	Echothiophate Dose	Outcome	Total LD₅₀ Survived
		*U/ml*
1	Ad-control	1.2	2× LD₅₀	Died 13 min	N.A.
2	Ad-control	1.0	2× LD₅₀	Died 8 min	N.A.
3	Ad-control	1.3	2× LD₅₀	Died 3 min	N.A.
4	Ad-control	1.3	2× LD₅₀	Died 2 min	N.A.
5	Ad-MoBChE	2395.6	2× LD₅₀	Survived	19
			5× LD₅₀	Survived
			12× LD₅₀	Survived
			9× LD₅₀	Died 6 min
6	Ad-MoBChE	405.3	2× LD₅₀	Survived	4
			2× LD₅₀	Survived
			12× LD₅₀	Died 3 min
7	Ad-MoBChE	586.5	2× LD₅₀	Survived	4
			2× LD₅₀	Survived
			12× LD₅₀	Died 3 min
8	Ad-MoBChE	5897.1	2× LD₅₀	Survived	30
			5× LD₅₀	Survived
			12× LD₅₀	Survived
			11× LD₅₀	Survived^*
9	Ad-MoBChE	878.2	2× LD₅₀	Survived	5
			3× LD₅₀	Survived
			12× LD₅₀	Died 4 min
10	Ad-MoBChE	5717.6	2× LD₅₀	Survived	30
			5× LD₅₀	Survived
			12× LD₅₀	Survived
			11× LD₅₀	Survived^*

N.A., not applicable.
↵* These animals were alive and symptom-free 8 days after last dosing.