TABLE 2

GABAAR modulators enhance L-VGCC current decay

Diltiazem (30 and 10 μM) was analyzed for Cav1.2 and Cav1.3, respectively. Pentobarbital (0.3 and 1 mM) was analyzed for Cav1.2 and Cav1.3, respectively. r200 is the ratio of current remaining after a 200-ms depolarization relative to the peak current. Control r200 values were obtained in the same cells in the absence of drug. Per. Dec. represents average percentage drug-induced decrease in the r200 value.

DrugCav1.2Cav1.3
CON r200Drug r200Per. Dec.nCON r200Drug r200Per. Dec.n
%%
30/10 μM Diltiazem0.53 ± 0.030.45 ± 0.0615 ± 730.73 ± 0.060.76 ± 0.05−5 ± 44
30 μM Diazepam0.49 ± 0.090.26 ± 0.08**50 ± 740.68 ± 0.070.49 ± 0.07*28 ± 53
30 μM Flurazepam0.35 ± 0.090.25 ± 0.07*31 ± 440.79 ± 0.070.64 ± 0.1019 ± 63
30 μM Desalkylflurazepam0.36 ± 0.080.26 ± 0.07*30 ± 530.49 ± 0.070.35 ± 0.08**32 ± 76
30 μM Allopregnanolone0.50 ± 0.080.24 ± 0.06***56 ± 550.66 ± 0.110.57 ± 0.11*15 ± 33
0.3/1 mM Pentobarbital0.44 ± 0.100.19 ± 0.06*58 ± 530.69 ± 0.090.37 ± 0.11**49 ± 84
300 mM Ethanol0.29 ± 0.120.12 ± 0.0562 ± 430.54 ± 0.040.45 ± 0.04**16 ± 23

  • ↵* P < 0.05, drug versus CON r200, paired t test.

  • ↵** P < 0.01, drug versus CON r200, paired t test.

  • ↵*** P < 0.001, drug versus CON r200, paired t test.