Effect of superphysiological high-dose prednisolone on hepatic vitamin B6 profile and B6-metabolizing enzyme activity and gene expression
Prednisolone dose was 10 mg/kg/day via gastric gavage for 35 days. Data are presented as means ± S.D. Percentage changes and P values were calculated compared with controls. Bold values indicate data significantly different when compared to controls (P<0.05) in Table 1.
| Control (n = 6) | Prednisolone (n = 6) | P Value | % Change | |
|---|---|---|---|---|
| Plasma profile (nmol/l) | ||||
| PLP | 742.8 ± 164.1 | 1016.2 ± 189.2 | 0.01 | +36.8 ± 25.5 |
| PL | 201.8 ± 61.1 | 172.6 ± 40.0 | 0.36 | −14.5 ± 19.8 |
| 4-PA | 42.3 ± 11.3 | 68.9 ± 24.7 | 0.05 | +59.0 ± 57.0 |
| Liver B6 status | ||||
| B6 vitamer (nmol/g liver) | ||||
| PMP | 22.0 ± 3.0 | 24.2 ± 3.9 | 0.47 | 10.0 ± 17.6 |
| PLP | 19.0 ± 4.7 | 16.5 ± 3.2 | 0.47 | −13.2 ± 16.7 |
| PL | 1.1 ± 0.9 | 0.6 ± 0.3 | 1.00 | −50.2 ± 23.1 |
| Gene expression | ||||
| PDXP/18S | 0.22 ± 0.14 | 4.14 ± 2.82 | 0.01 | +1799 ± 1293 |
| PDXK/18S | 0.32 ± 0.11 | 0.94 ± 0.40 | 0.01 | +196.2 ± 126.4 |
| PMPO/18S | 0.38 ± 0.31 | 0.74 ± 0.53 | 0.29 | 93.6 ± 138.3 |
| Enzyme activity (nmol/h · mg) | ||||
| PDXP | 325.0 ± 25.7 | 299.0 ± 25.8 | 0.06 | −8.1 ± 7.9 |
| PDXK | 9.2 ± 2.2 | 15.9 ± 4.3 | 0.02 | +72.7 ± 46.1 |
| PMPO | 1.4 ± 0.2 | 1.5 ± 0.1 | 0.58 | 6.1 ± 6.8 |